The next step, after we have acquired our two images (whether from a camera, confocal microscope, computer program, or other source) is to prepare them for processing. Remember that, for the most part, color images don't make good anaglyphs. While the two images we're using are an exception, we'll create a more conventional greyscale-based anaglyph anyway.

To do this, we need to remove the color data from the images without removing the color channels. (Simply converting the images to greyscale would leave them with only a single "Black" channel and therefore no way to perform later steps.) Fortunately, Photoshop includes a Desaturate command for just this purpose. In this way, we can strip all color data from an image without removing the separate channels for red, green, and blue.

If the image you are starting with is already a greyscale image, simply select Image > Mode > RGB. Otherwise:

  1. From the Image menu, select the Adjustments submenu and finally the Desaturate command.
  2. Photoshop will strip the colors from the image while leaving the image in RGB mode. This will be necessary for the following step, which is why we did not simply select Grayscale from the Mode menu.

Sometimes, images may be dim or too saturated. However, we will fix this in a later step.

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